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Our three‑pronged approach demonstrates that relying solely on metadata can miss up to of relevant records. Sequence‑based BLAST, complemented by fuzzy identifier matching, proved essential for comprehensive recovery. The pipeline we present can be adapted to any viral organism where “dark” entries exist.
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The retrieved sequences (n = 27) were aligned with reference HDV genotypes (1–8) using MAFFT (v7.525) with the --auto setting. Ambiguously aligned ends were trimmed with trimAl (v1.4). A maximum‑likelihood tree was inferred with IQ‑TREE (v2.2.2) employing the best‑fit nucleotide substitution model (selected by ModelFinder). Node support was assessed via 1,000 ultrafast bootstrap replicates. Do you need help configuring